The supplement played a large role in the controversial non-fiction book Game of Shadows by Mark Fainaru-Wada and Lance Williams. It is important to note that “of the 57 Western Washington University football players who signed up for the study, 30 quit. By the end, only a dozen players were using ZMA while 15 took the placebo.” (Game Of Shadows 46)[5]
A 1999 study was undertaken on NCAA Football players during an 8 week spring training program. The control group was told to cease taking any nutritional supplements. Those who took the ZMA tablets showed greater increases in muscle strength, free testosterone levels, and IGF-1 levels.[3] This study was funded by SNAC Systems Inc. (the patent holders) and one of the study’s authors (Victor Conte) has equity in this company.
Another study in 2004 conducted jointly by the Exercise & Sport Nutrition Lab of Baylor University, IMAGINutrition, and the Journal of the International Society of Sports Nutrition, found that ZMA has no effect on strength, hormone levels, or anaerobic capacity.[4]
ZMA (Zinc monomethionine aspartate and Magnesium Aspartate) is a supplement used by bodybuilders and athletes. It was developed by Victor Conte (founder of BALCO Laboratories in Burlingame, California) and is a combination of zinc, magnesium and vitamin B6. The formula is “patent pending” and the name “ZMA” trademarked by SNAC System Inc, also founded by Victor Conte. ZMA is claimed to raise testosterone and IGF-1 levels which may aid in gaining muscle size and strength.
ZMA is a combination of two minerals, zinc and magnesium, and Vitamin B-6 or pyridoxine. All three of these compounds are important in biological processes, and while studies have shown that most Americans get enough zinc and Vitamin B6,[1] more than 50% are deficient in magnesium.[2]
An increase in exercise can lead to the loss of vitamins and minerals making it particularly important for bodybuilding due to the blood sugar level rises and urination increases, increasing the loss of magnesium, zinc, B12, B6, folic acid, and many other nutrients. Although drinking water re-hydrates an athlete, fruit juice, sports drinks or foods high in water such as vegetables are needed to replenish water-soluble nutrients.
The proportion of ingredients generally used in products is 20-30 mg Zinc, 400-500 mg Magnesium and ~10mg B6. According to the label directions, ZMA should be taken before bed on an empty stomach (2 hours after eating your last meal and at least 30 minutes prior to any other supplements). The product should not be taken with calcium (cheese,milk,etc.), the reason being that calcium blocks the absorption of zinc.
More than other protein supplements, whey protein powder is commonly used by bodybuilders and other athletes to accelerate muscle development and aid in recovery. Some individuals with suppressed or otherwise abnormal immune systems or degenerative diseases use undenatured bioactive whey proteins to increase their antioxidant levels. These native whey proteins are a good source of cysteine, a conditionally essential amino acid, which is the rate-limiting factor for the body’s production of glutathione[1], an important antioxidant.
In a study, it was found that whey supplements may prevent blood sugar spikes after high-carbohydrate meals, which is useful for diabetics.[2]
Whey protein typically comes in three major forms: concentrate, isolate, and hydrolysate.
Concentrates contain a low level of fat and cholesterol but, in general, have higher levels of bioactive compounds, and carbohydrates in the form of lactose — they are 29%-89% protein by weight.
Isolates are processed to remove the fat, and lactose, but are usually lower in bioactive compounds as well — they are 90%+ protein by weight. Both of these types are mild to slightly milky in taste.
Hydrolysates are predigested, partially hydrolyzed whey proteins that, as a consequence, are more easily absorbed, but their cost is generally higher. Whey protein hydrolysate also tends to taste quite different than other forms of whey protein, usually in a way that many find undesirable but can be masked when used in beverages.[citation needed]
Whey protein can be denatured by heat. High heat (like the sustained high temperatures above 72 degrees Celsius (160 degrees Fahrenheit) associated with the pasteurization process) denatures whey proteins, destroying some bioactive compounds, such as the amino acid cysteine. While native whey protein does not aggregate upon renneting or acidification of milk, denaturing the whey protein triggers hydrophobic interactions with other proteins, and the formation of disulfide bonds between whey proteins and casein micelles, leading to aggregation with other milk proteins at low pH.
Whey protein is the collection of globular proteins that can be isolated from whey, a by-product of cheese manufactured from cow’s milk. It is typically a mixture of beta-lactoglobulin (~65%), alpha-lactalbumin (~25%), and serum albumin (~8%), which are soluble in their native forms, independent of pH. Whey has the highest biological value (BV) of any known protein.
The protein fraction in whey (approximately 10% of the total dry solids within whey) comprises four major protein fractions and six minor protein fractions. The major protein fractions in whey are beta-lactoglobulin, alpha-lactalbumin, bovine serum albumin and immunoglobulins. Each of these components has important disease-fighting effects.[citation needed] In addition, whey protein is easily digestible.
Two alkaloids that seem to cause limb paresis (staggers) in sheep that eat Tribulus terrestulis are the beta-carbolines harman (harmane) and norharman (norharmane).[13] The alkaloid content of dried foliage is about 44 mg/kg.[13]
Two weevils, Microlarinus lareynii and M. lypriformis, native to India, France, and Italy, were introduced into the United States as biocontrol agents in 1961. Both species of weevils are available for purchase from biological suppliers but purchase and release is not often recommended because weevils collected from other areas may not survive at the purchaser’s location.
Microlarinus lareynii is a seed weevil that deposits its eggs in the young burr or flower bud and the larvae feed on and destroy the seeds before they pupate, emerge, disperse, and start the cycle over again. Its life cycle time is 19 to 24 days. Microlarinus lypriformis is a stem weevil that has a similar life cycle, excepting the location of the eggs, which includes the undersides of stems, branches, and the root crown. The larvae tunnel in the pith where they feed and pupate. Adults of both species overwinter in plant debris. Although the stem weevil is slightly more effective than the seed weevil when each is used alone, the weevils are most effective if used together and the puncture vine is moisture-stressed.
Chemical control is generally recommended for home control of puncture vine. There are few preemergent herbicides that are effective. Products containing oryzalin, benefin, or trifluralin will provide partial control of germinating seeds. These must be applied prior to germination (late winter to midspring).
After plants have emerged from the soil (postemergent), products containing 2,4-D, glyphosate, and dicamba are effective on puncture vine. Like most postemergents they are more effectively maintained when caught small and young. Dicamba and 2,4-D will cause harm to most broad-leaved plants so the user should take care to avoid over-application. They can be applied to lawns without injuring the desired grass. Glyphosate will kill or injure most plants so it should only be used as spot treatments or on solid stands of the weed.